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  • br Dopa s release rate was observed The solubility

    2020-08-02


    Dopa's release rate was observed. The solubility of L-Dopa is mainly influenced by the pH of the media. The decrease in the pH of an aqu-eous solution leads to increasing the solubility of L-Dopa. Therefore, L-Dopa released from [email protected] much faster in lower pHs. Also, reducing the pH of the aqueous solution causes weakening the drug/ carrier interactions due to the protonation of amino and hydroxyl groups, and therefore the amount of L-Dopa release enhanced. The rate of drug release from the carrier was slow in high pHs and the con-centration of total released L-Dopa was evaluated as 53.68%. So, it could be resulted that [email protected] can act as an ideal pH-re-sponsive carrier for L-Dopa.
    3.3. Therapeutic investigation
    Interestingly, L-Dopa and [email protected]@L-Dopa induced Salvinorin A death in Mel-Rm cells. To checking this possibility, cells were exposed to different concentrations of L-Dopa and [email protected]@L-Dopa, afterward, cell viability was measured by trypan blue assay at 24 h after the exposure (Fig. 15). In L-Dopa group, the results of this experiment showed that in treatments 1–6 the percentage of cell viability was de-creased compared with control cells (p < 0.05). In [email protected] group, exposure of the cells to the different concentrations of pure nanoparticle was decreased the cell viability in treatments 5 and 6 compared with control cells (p < 0.05). The percentage of cell viabi-lity was decreased in treatments 5 and 6 compared with treatments 1–3 (p < 0.05). In [email protected]@L-Dopa group, exposure of the cells to the different concentrations of nano-drug caused the decrement of cell viability in treatments 1–6 compared with control cells (p < 0.05). The percentage of cell viability was decreased in treatment 6 compared with treatments 1–4 (p < 0.05). The percentage of cell viability was decreased in each treatment of [email protected]@L-Dopa compared with the same treatments in L-Dopa treatment, respectively (p < 0.05) [1].
    Interestingly, L-Dopa and [email protected]@L-Dopa groups in-duced cells death in Mel-Rm cells. To check the cytotoxic effect possi-bility, cells were exposed to different concentrations of L-Dopa and [email protected]@L-Dopa groups, then, cell cytotoxicity was mea-sured by LDH colorimetric assay at 24 h after the exposure (Fig. 16). In L-Dopa group, the results of this experiment showed that in treatments 1–6 the percentage of cell cytotoxicity was increased compared with control cells (p < 0.05). In [email protected] group, exposure of the
    N. Shahabadi, et al.
    cells to the different concentrations of pure nano-particle was increased the cell cytotoxicity in treatments 5 and 6 compared with control cells (p < 0.05). The percentage of cell cytotoxicity was increased in treatments 5 and 6 compared with treatments 1–3 (p < 0.05). In [email protected]@L-Dopa group, exposure of the cells to the different concentrations of nano-drug was increased the cell cytotoxicity in treatments 1–6 compared with control cells (p < 0.05). The percen-tage of cell cytotoxicity was increased in treatment 6 compared with treatments 1–4 (p < 0.05). The percentage of cell cytotoxicity was increased in each treatment of [email protected]@L-Dopa compared with the same treatments in L-Dopa treatment, respectively (p < 0.05).
    3.3.3. Cell proliferation inhibition and IC50 values
    The inhibitory effect of the agents on the proliferation of the Mel-Rm cell lines was evaluated and the dose-response curves are depicted in Fig. 17. After 24 h, Cell proliferation in all treatments was decreased as dose depended compared with control cells (p < 0.05). In L-Dopa group IC50 value was evaluated to be 5.48 μM. In [email protected]@L-Dopa group, the percentage of cell proliferation was about 100%. The cell proliferation in all treatments was decreased compared with control cells (p < 0.05). In treatments 1–6, cell viability was decreased and cell cytotoxicity was increased compared with intra-control treatment and parallel treatments in L-Dopa group, respectively (p < 0.05). In [email protected]@L-Dopa group, IC50 was 3.87 μM. Another important effect becomes apparent when analyzing Fig. 17, which summarized in Table 3; L-Dopa-loaded [email protected] nanoparticles displays a more pronounced anticancer activity than L-Dopa alone. It means that the nanoencapsulation of L-Dopa in [email protected] nanoparticles leads to a significant enhancement of the biological effect of L-Dopa. This
    Fig. 15. The cell viability of Mel-Rm cells in different treatment media.
    There were three Groups: Group I: incubated with L-Dopa,
    Group II: incubated with [email protected]@L-Dopa, and Group III: incubated with [email protected] There were control and six treatments in each group, including; control: 0.0 μM, treatment 1:1 μM, treatment 2:2 μM, treatment 3:4 μM, treatment 4:8 μM, treatment 5:16 μM, and treatment 6:32 μM of material in each groups, re-spectively. All data represented by mean ± S.E.M (p < 0.05). Significant difference between treatments and control treatment in each group; *p < 0.05; one way ANOVA for repeated measures.